扩展器
精液
脂质过氧化
DNA断裂
精液质量
精子
男科
顶体
化学
番红花苷
生物化学
生物
抗氧化剂
医学
细胞凋亡
有机化学
程序性细胞死亡
聚氨酯
作者
Alfonso Calabria,Chiara Del Prete,Ciarcia Roberto,V. Longobardi,Stefano Spada,Maria Teresa Alfano,Daniela De Felice,B. Gasparrini,N Cocchia
标识
DOI:10.1016/j.anireprosci.2023.107374
摘要
The aim of the study was to evaluate the effects of crocin on canine sperm quality parameters during prolonged storage at 4°C. Ejaculates from 10 dogs were diluted in a TRIS- egg yolk extender supplemented with 0 (control group), 0.5, 1, and 2 mM crocin and stored at 4 °C. Sperm membrane functional integrity, motility, and kinetics were assessed after 3 h, 24 h, 4 days and 7 days of storage. Based on the results, the more efficient concentration of crocin (0.5 mM) was chosen to evaluate sperm intracellular ROS levels, lipid peroxidation, and DNA fragmentation vs. the control. Semen with the addition of 0.5 mM crocin with respect to the control exhibited: i) increased (P<0.05) sperm membrane functionality at 4 and 7 days of storage; ii) higher (P <0.05) average path (VAP), straight-line velocities (VSL), and beat cross frequency (BCF) at 4 d of storage at 4°C; iii) decreased (P <0.05) intracellular ROS levels after 3 and 24 h storage. No differences in lipid peroxidation and DNA fragmentation were recorded between the control and C0.5 groups at any time point. Lipid peroxidation did not increase over time, while DNA fragmentation increased (P <0.05) in both groups after 4 days of storage. The results demonstrated that the enrichment of extender with crocin improves to a certain extent canine semen quality, particularly after 4 days of storage at 4 °C.
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