In Vivo Time-Lapse Imaging of Zebrafish Embryonic Development

INTRODUCTION Intravital time-lapse imaging is a powerful technique for investigating continuous developmental processes without missing crucial events. Because of the rapid embryogenesis, external development, and transparency of zebrafish embryos, their developmental processes can be visualized in time-lapse studies in the context of the living organism. The following protocol describes a method for performing intravital time-lapse imaging of zebrafish embryos over several days using confocal or compound stereomicroscopes.