酵母
生物
计算生物学
酿酒酵母
人性化鼠标
定向进化
基因
生物技术
突变体
生物化学
体内
作者
Farzin Roohvand,Parastoo Ehsani,Meghdad Abdollahpour‐Alitappeh,Mehdi Shokri,Niloufar Kossari
标识
DOI:10.1080/13543776.2020.1781816
摘要
Introduction Yeast humanization, ranging from a simple point mutation to substitution of yeast gene(s) or even a complete pathway by human counterparts has enormously expanded yeast biomedical applications.Areas covered General and patent-oriented insights into the application of native and humanized yeasts for production of human glycoproteins (gps) and antibodies (Abs), toxicity/mutagenicity assays, treatments of gastrointestinal (GI) disorders and potential drug delivery as a probiotic (with emphasis on Saccharomyces bulardii) and studies on human diseases/cancers and screening effective drugs.Expert opinion Humanized yeasts cover the classical advantageous features of a ‘microbial eukaryote’ together with advanced human cellular processes. These unique characteristics would permit their use in the production of functional and stable therapeutic gps and Abs in lower prices compared to mammalian (CHO) production-based systems. Availability of yeasts humanized for cytochrome P450 s will expand their application in metabolism-related chemical toxicity assays. Engineered S. bulardii for expression of human proteins might expand its application by synergistically combining the probiotic activity with the treatment of metabolic diseases such as phenylketonuria via GI-delivery. Yeast models of human diseases will facilitate rapid functional/phenotypic characterization of the disease-producing mutant genes and screening of the therapeutic compounds using yeast-based high-throughput research techniques (Yeast one/two hybrid systems) and viability assays.
科研通智能强力驱动
Strongly Powered by AbleSci AI