DPPH
菊花
化学
凉茶
抗氧化剂
高效薄层色谱法
绿原酸
色谱法
传统医学
薄层色谱法
植物
生物化学
生物
医学
作者
Shing Chung Lam,Sio-Fong Lam,Jing Zhao,Shao‐Ping Li
标识
DOI:10.1111/1750-3841.13402
摘要
Abstract A simple and efficient method based on high‐performance thin‐layer chromatography coupled with 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) bioautography (HPTLC‐DPPH) was established for the screening and comparison of antioxidants in different parts of Coreopsis tinctoria herbal tea from different origins and other related herbal tea materials, which used Chrysanthemum morifolium cv. “ Gongju ” and “ Hangju ” in this study. Scanning densitometry after DPPH derivatization was applied for the determination of antioxidant capacities of isolated compounds in each sample. It is considered that ethanol extracts of C. tinctoria had stronger antioxidant activity and more characteristic bands than those of 2 compared samples, C. morifolium cv. “ Gongju ” and “ Hangju .” Chemometric analysis results showed that the combination of hierarchical clustering analysis and principal component analysis based on determined antioxidant capacities could be used for the discrimination of different parts of C. tinctoria and C. morifolium . Results showed that 7 compounds made up the major contributions of antioxidant activity in C. tinctoria , including okanin, isookanin, marein, flavanomarein, 5,7,3′,5′‐tetrahydroxyflavanone‐7‐ O ‐glucoside, 3,5‐dicaffeoylquinic acid, and chlorogenic acid. Therefore, 7 compounds were identified as major antioxidant biomarkers for quality control of C. tinctoria . Results demonstrated that the established method could be applied for the identification of C. tinctoria , and were beneficial for the bioactivity‐based quality control of C. tinctoria .
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