色谱法
化学
芦荟大黄素
高效液相色谱法
检出限
二氯甲烷
萃取(化学)
醋酸
药代动力学
大黄素
溶剂
生物化学
医学
内科学
有机化学
作者
Marco Zaffaroni,Carla Mucignat‐Caretta,Teresa Pecere,Giuseppe Zagotto,Roberta Frapolli,Maurizio D’Incalci,Massimo Zucchetti
标识
DOI:10.1016/j.jchromb.2003.08.012
摘要
An isocratic high-performance liquid chromatography (HPLC) method was developed and validated to determine Aloe Emodin (AE) in mouse plasma. The analysis required 0.3 ml of plasma and involves extraction with dichloromethane. The HPLC separation was carried out on Symmetry Shield RP18, a mobile phase of methanol-water-acetic acid (65:35:0.2) and fluorescence detection at lambda(ex)=410 nm and lambda(em)=510 nm. The retention time of AE was 11.7 min. The assay was linear from 10 to 1,000 ng/ml (r2 > or = 0.999), showed intra- and inter-day precision within 7.8 and 4.7%, and accuracy of 87.3-105.7%. Detection limit (LOD) and quantification limit (LOQ) were 4.5 and 5 ng/ml, respectively. The method was applied to determine for the first time the pharmacokinetic of AE in mice.
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