壳聚糖
再生(生物学)
Zeta电位
脚手架
吸附
材料科学
骨愈合
抗压强度
蛋白质吸附
组织工程
生物医学工程
间充质干细胞
化学
复合数
骨组织
化学工程
复合材料
纳米技术
外科
有机化学
细胞生物学
纳米颗粒
工程类
医学
生物
作者
Xiangyu Wang,Yufang Li,Wenjuan Ren,Ruxia Hou,Haifeng Liu,Ran Li,Shaofu Du,Lu Wang,Junyu Liu
标识
DOI:10.1080/09205063.2021.1916868
摘要
The bone healing defects resulting from bone disease remain a significant clinical challenge. The bone tissue engineering scaffolds combined with osteoinductive compounds represent an effective approach to overcome this challenge. In this study, a novel chitosan-based scaffold was prepared by incorporating modified natural diatomite (DE) as filler and adsorption element. Specifically, modified-diatomite (MDE) was synthesized by grafting polyethyleneimine (PEI) on the surface of diatomite via hydroxyl groups. The physicochemical characteristics of MDE, including chemical composition, zeta potential, and adsorption behavior, were investigated successively. Further, the mechanical strength, drug release, cytotoxicity and osteogenic activity analyses were carried out for the scaffold material. The FTIR and zeta potential analyses exhibited that the amino groups (–NH2) were grafted on MDE, and the surface potential of diatomite altered from −24 mV to 55 mV. Subsequently, the protein adsorption capacity and cytocompatibility of MDE were observed to be improved as compared to DE. The compressive strength was observed to be enhanced due to the addition of MDE. Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity. The findings obtained in this study reveal that the MDE-rhBMP-2/CS composite scaffold can be potentially used to promote the bone tissue regeneration.
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