Exploring of Counting Method of Mouse Blastocyst and Hatched Blastocyst

The experiment was blastocyst and hatch blastocyst of ICR mouse culture in vitro, and the effect of two methods to the cell counting of mouse embryo was compared. Method 1 was that blastocyst and hatch blastocyst was pressed with the 0.5% low osmosis liquid of Sodium Citrate and Giemsa dye, and the treat time of low osmosis and dye of corresponding embryo was identical in different period;Method 2 was blastocyst and hatched blastocyst was pressed with Hematoxylin, and the treat time was different in different period.The results indicated that in method 2 the outline of mouse blastocyst and hatched blastocyst cell ball was clear, the cleft of cell was obvious, its rate of observation was 94.7% and 96.8% respectively; while in method 1 the cleft of embryo cell ball was unclear, and the outline of some cells was blurry, and its rate was 70.5% and 69.6% respectively.Method 2 is significantly better than method 1 by adopting count method of press of small mouse blastocyst and hatched blastocyst ( P0.05) .