Flavones of Vernonia spirei Gandog

The chemical constituents of Vernonia spirei Gandog were studied.The constituents were separated and purified from MeOH extract of the whole plant with column chromatographic methods through silica gel,RP-18 silica gel,Sephadex LH-20,HPLC,and preparative TLC.Their chemical structures were characterized by spectroscopic methods including NMR and EIMS.The result showed that Vernonia spirei Gandog contained two components including 5,7,4'-trihydroxy-6,3'-dimethoxyflavone(Ⅰ),5,3'-dihydroxy-6,7,4'-trimethoxyflavone(Ⅱ).These compounds were obtained from this genus for the first time.