The polysaccharides from Gomphidius rutilus was extracted with hot water extraction method.The effect of extraction times,extraction temperature,extraction time and ratio of water to raw material on optimization extraction of polysaccharide were studied by single factor experiment and orthogonal experiment.The results showed that the best extraction conditions were chosen as:times of extraction was 3,temperature of extraction 100℃,time of extraction 2 h and ratio of water to raw material 1∶16.Under the optimize conditions,the eventually yield of polysaccharides from Gomphidius rutilus reached(7.64±0.15)%.