Rapid Determination of Hydroxysafflor Yellow A Content in Countercurrent Extracting Process of Carthamus Tinctorius L. by Near Infrared Spectroscopy

Near infrared spectroscopy(NIRS) has become a very popular technique for non-invasive assessment of the active components in traditional Chinese medicine.The feasibility of using NIR technology for rapid quantifying hydroxysafflor yellow A(HSYA) in countercurrent extracting of Carthamus tinctorius L.was investigated by means of spectral transmittance mode.HPLC was used as the reference method to determine the content of HSYA and multivariate calibration models based on PLS algorithm were developed to correlate the spectra and the corresponding values determined by the reference method.The samples in the process of extraction,which did not participate in modeling were used to test the performance of the models.The correlation coefficient of the calibration model was 0.982,and the predicted coefficient was 0.965.Root mean square error of calibration(RMSEC) and root mean square error of prediction(RMSEP) for them were 0.0021 and 0.0030,and the relative standard error of calibration(RSEC) and relative standard error of prediction(RSEP) were 3.960% and 5.252%,respectively.It was concluded that the proposed method is fast and nondestructive,and it provides a novel efficient and environmental friendly approach for the fast determination of the active components in traditional Chinese medicine for countercurrent extracting process analysis.