UV spectrophotometric determination of acetylation substitution degree in Konjac glucomannan

Objective:To establish a method for the determination of acetylation substitution degree in Konjac glucomannan.Methods:β-D-Glucose pentaacetate was used as the reference component for UV spectrophotometric,detemined at 500 nm.Results:The range of calibration curve was between 15.4-92.4 μg·mL-1(r=0.9996),and the detection limit was 11.1 μg·mL-1.The average recovery was 101.6%(n=9).The precision(RSD) was 0.08%.Conclusion:The method is simple,rapid,accurate with good reproducibility for determining the degree of acetylation substitution of Konjac glucomannan.