Screening of Antifungal Lactic Acid Bacteria and its Application in Chinese Steamed Bread and Yogurt

With the overlay method,the strain IMAU80134 with a high antifungal activity against Penicillium roqueforti was screened from 140 Lactobacillus plantarum. Lactobacillus plantarum TK9 isolated from acid gruel displayed a high antifungal activity against Aspergillus flavus. The broad spectrum of antifungal activity of the 2 colonies indicated that both L. plantarum TK9 and IMAU80134 could inhibit Penicillium citrinum,Penicillium oxalicum and Aspergillus fumigatus,in addition to A. flavus and P. roqueforti. L. plantarum TK9 and IMAU80134 had stronger antifungal activity against P.oxalicum,The inhibition zone radii were 12.3 mm and 12.7 mm respectively. Meanwhile,both L. plantarum TK9 and IMAU80134 were applied to the bio-preservation of Chinese steamed bread and yoghurt in vitro simulation experiments.Their shelf-life could be extended 1 and 4 days respectively by L. plantarum TK9,3 and 4 days respectively by L.plantarum IMAU80134.