The study on aprotinin blunt inflammation induced by Cardiopulmonary bypass in Children

Objective: To evaluated the inhibitory effects of aprotinin on plasma TNF α, IL 6, IL 8 during and after cardiopulmonary bypass in children. Methods: 19 children with congenital heart disease were randomized to aprotinin group (n=10) or control group (n=9). Blood samples were collected during and after cardiopulmonary bypass in each group. Cytokines including tumor necrosis factor α(TNF α), IL 6 and IL 8 were measured respectively by ELISA (enzyme linked immunosorbent assay) technique with matched antibody pairs. Results: In control group, TNF α,IL 6 and IL 8 were significantly increased during and early after CPB. IL 8 and TNF α were returned to normal 24 hours after operation. No significant changes were noted in TNF α in aprotinin group. In comparison with control group, the increasing degree of IL 6 and IL 8 were significantly low. (P0 05 or P0 01). Conclusion: Aprotinin may inhibit cardiopulmonary bypass induced inflammation by the suppressing cytokine production such as TNF α, IL 6 and IL 8.