Rapid Determination of Ferulic Acid and Linolic Acid in Angelica sinensis by Near-Infrared Diffuse Reflectance Spectroscopy

The NIR spectra of 145 batches of Angelica sinensis were collected by near-infrared diffuse reflectance spectroscopy. With the contents of ferulic acid and linolic acid determined by RP-HPLC as reference,the PLS( partial least squares) quantitative analysis model was established by TQ Analyst 8. 0 software to rapidly and accurately determine the contents of ferulic acid and linolic acid in A. sinensis. The correlation coefficient,RMSEC( the root-mean-square error of calibration) and RMSEP( the root-mean-square error of prediction) of ferulic acid of the calibration set reached 0.9721,0. 5942 and 0. 6747,respectively. The correlation coefficient,RMSEC and RMSEP of linolic acid of the calibration set reached 0. 9673,0. 4573 and 1. 0682,respectively. The average recoveries of ferulic acid and linolic acid of validation set were 101. 98% and 102. 03%,respectively. The established method was simple and rapid to operate,and the result by the prediction model was accurate and reliable. Hence,it can be routinely used for the content determination of ferulic acid and linolic acid in A. sinensis.