组氨酸
色氨酸
苯丙氨酸
拉曼光谱
化学
谱线
氨基酸
酪氨酸
拉曼散射
芳香族氨基酸
激发
分析化学(期刊)
有机化学
光学
生物化学
电气工程
物理
工程类
天文
作者
Fani Madzharova,Zsuzsanna Heiner,Janina Kneipp
标识
DOI:10.1021/acs.jpcc.6b10905
摘要
In this work, we report nonresonant surface-enhanced hyper-Raman (SEHRS) spectra of the amino acids tryptophan, histidine, phenylalanine, and tyrosine using silver nanoparticles. The spectra are obtained at an excitation wavelength of 1064 nm and compared to the corresponding surface-enhanced Raman scattering (SERS) spectra measured at 532 nm excitation. The majority of the bands in the SEHRS spectra are assigned. Important hallmarks of the spectra include strongly diminished or absent bands from the ring breathing modes. SEHRS and SERS spectra obtained from histidine and tyrosine indicate changes at slightly varied amino acid concentration. Small changes in the SEHRS spectra were more pronounced than variation in the corresponding SERS data, supporting the high sensitivity of the SEHRS spectra with respect to structural changes due to small variations in surface environment. The possibility to measure nonresonant SEHRS spectra of amino acids in solution and the complementary information obtained from the spectra demonstrates the potential of this method for future investigations of proteins and more complicated biological structures and their interaction with nanostructures.
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