磷
氮气
反硝化
硝化作用
铵
硝酸盐
环境化学
亚硝酸盐
微生物学
废水
好氧反硝化
化学
活性污泥
生物
环境工程
反硝化细菌
环境科学
有机化学
作者
Weilai Fu,Youxi Zhao,Qiang Wang,Yang Xuan,Ziyang Song,Peifeng Duan,Meijuan Xu,Xian Zhang,Zhiming Rao
标识
DOI:10.1016/j.biortech.2023.129922
摘要
A novel strain having ability to simultaneously remove ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and phosphorus was isolated from swine farm wastewater and was identified as Raoultella ornithinolytica YX-4 (NCBI accession number: OR646540). Nitrogen and phosphorus balance analysis combined with amplification of key enzyme genes of metabolic pathways revealed that the strain possess heterotrophic nitrification, aerobic denitrification, phosphorus accumulation and assimilation pathways. Significant removal of ammonium nitrogen, nitrate nitrogen and nitrite nitrogen were achieved (99%, 97% and 93% respectively) with optimal culture conditions. The transcript level of key enzyme genes was detected at different incubation period, and significant up-regulation of glnA, narI, narH, nirB, nirD, ppk1, and ppk2 was noted. This is the first report of the denitrification of phosphorus accumulating organisms R. ornithinolytica and reveals tangible results of key enzyme gene expression during real wastewater treatment.
科研通智能强力驱动
Strongly Powered by AbleSci AI