下调和上调
细胞外基质
间充质干细胞
细胞生物学
肌腱
再生医学
间质细胞
化学
生物
癌症研究
干细胞
解剖
生物化学
基因
作者
Angela Papalamprou,Victoria Yu,Angel Chen,Tina Stefanovic,Giselle Kaneda,Khosrowdad Salehi,Chloe Castaneda,Arkadiusz Gertych,Juliane D. Glaeser,Dmitriy Sheyn
摘要
Regenerative therapies for tendon are falling behind other tissues due to the lack of an appropriate and potent cell therapeutic candidate. This study aimed to induce tenogenesis using stable Scleraxis (Scx) overexpression in combination with uniaxial mechanical stretch of iPSC-derived mesenchymal stromal-like cells (iMSCs). Scx is the single direct molecular regulator of tendon differentiation known to date. Bone marrow-derived (BM-)MSCs were used as reference. Scx overexpression alone resulted in significantly higher upregulation of tenogenic markers in iMSCs compared to BM-MSCs. Mechanoregulation is known to be a central element guiding tendon development and healing. Mechanical stimulation combined with Scx overexpression resulted in morphometric and cytoskeleton-related changes, upregulation of early and late tendon markers, and increased extracellular matrix deposition and alignment, and tenomodulin perinuclear localization in iMSCs. Our findings suggest that these cells can be differentiated into tenocytes and might be a better candidate for tendon cell therapy applications than BM-MSCs.
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