化学
受体
生物物理学
受体酪氨酸激酶
细胞生物学
磷酸化
细胞膜
细胞表面受体
细胞信号
信号转导
细胞
DNA
纳米技术
生物化学
生物
材料科学
作者
Jin Wang,Juan Song,Xian Zhang,Shumin Wang,Bin Kang,Xiangling Li,Hong‐Yuan Chen,Jing‐Juan Xu
摘要
Decrypting the dynamics of receptor dimerization on cell membranes bears great importance in identifying the mechanisms regulating diverse cellular activities. In this regard, long-term monitoring of single-molecule behavior during receptor dimerization allows deepening insight into the dimerization process and tracking of the behavior of individual receptors, yet this remains to be realized. Herein, real-time observation of the receptor tyrosine kinases family (RTKs) at single-molecule level based on plasmon rulers was achieved for the first time, which enabled precise regulation and dynamic monitoring of the dimerization process by DNA programming with excellent photostability. Additionally, those nanoprobes demonstrated substantial application in the regulation of RTKs protein dimerization/phosphorylation and activation of downstream signaling pathways. The proposed nanoprobes hold considerable potential for elucidating the molecular mechanisms of single-receptor dimerization as well as the conformational transitions upon dimerization, providing a new paradigm for the precise manipulation and monitoring of specific single-receptor crosslink events in biological systems.
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