发起人
雅罗维亚
枯草芽孢杆菌
酿酒酵母
酵母
生物
基因
转录调控
生物制造
生物化学
化学
基因表达
遗传学
细菌
作者
Chang Wang,Minxin Lin,Zhiliang Yang,Xueyao Lu,Yingfang Liu,Huizhi Lu,Jiang Zhu,Xiao‐Man Sun,Yang Gu
标识
DOI:10.1016/j.procbio.2022.11.023
摘要
Yarrowia lipolytica, an oleaginous yeast with the GRAS status, has been developed as a platform for the chemical production. Promoter engineering is an important approach to regulating gene expression at the transcriptional level, which is of great significance in constructing microbial cell factories. Although some work on promoter study has been carried out in Y. lipolytica, obtained achievements still need further replenishment and development, compared explicitly with Saccharomyces cerevisiae, Bacillus subtilis, and other microorganisms. For this, we screened 81 endogenous promoters in Y. lipolytica, mainly involved in carbon metabolism and nitrogen metabolism. As a result, we obtained 15 strong promoters, 41 medium strength promoters, and 25 weak promoters, with the strength spanning from 0.06 % to 1.60-fold of PTEF promoter. Moreover, as a proof-of-concept, the identified promoter library was applied for expressing UDP-glycosyltransferase to produce salidroside, resulting in a production of 95.64 mg/l in shake flask, which is 3.92-times that of the parental strain. In general, our study provides a unique and available endogenous promoter library for Y. lipolytica.
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