寡霉素
ATP合酶
ATP酶
生物化学
化学
亚软骨颗粒
酶
化学渗透
反转运蛋白
缬霉素
三磷酸腺苷
二磷酸腺苷
腺嘌呤核苷酸
二价
电子传输链
离子运输机
Mersalyl公司
线粒体
生物物理学
核苷酸
生物
膜电位
膜
血小板
血小板聚集
有机化学
基因
免疫学
作者
Mi-Sun Kim,Eun-Sook Song
出处
期刊:Biochimie
[Elsevier]
日期:2010-04-01
卷期号:92 (4): 333-342
被引量:8
标识
DOI:10.1016/j.biochi.2010.01.014
摘要
In this work, we present evidence of Fe(2+) transport by rat heart mitochondrial F(1)F(0) ATP synthase. Iron uptake by the vesicles containing the enzyme was concentration- and temperature-dependent, with an optimum temperature of 37 degrees C. Both ATP and ADP stimulated iron uptake in a concentration-dependent manner, whereas AMP, AMPPCP, and mADP did not. Inhibitors of the enzyme, oligomycin, and resveratrol similarly blocked iron transport. The iron uptake was confirmed by inhibition using specific antibodies against the alpha, beta, and c subunits of the enzyme. Interestingly, slight transport of common divalent and trivalent metal ions such as Mg(+2), Ca(+2), Mn(+2), Zn(+2), Cu(+2), Fe(+3), and Al(+3) was observed. Moreover, Cu(+2), even in the nM range, inhibited iron uptake and attained maximum inhibition of approximately 56%. Inorganic phosphate (Pi) in the medium exerted an opposite effect depending on the type of adenosine nucleotide, which was suppressed with ATP, but enhanced with ADP. A similarly stimulating effect of ATP and ADP with an inverse effect of Pi suggests that the activity of ATPase and ATP synthase may be associated with iron uptake in a different manner, probably via antiport of H(+).
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