Streptomyces liliifuscus sp. nov and an anti-ginger plague agent Streptomyces liliiviolaceus sp. nov, two novel species isolated from soil of Lilium lancifolium

Two novel strains of actinobacteria, ZYC-3 T and BH-SS-21 T , were isolated from Hunan Province, PR China. The fermentation broth of BH-SS-21 T inhibited the rapid spread of ginger blast, unlike that of ZYC-3 T . The taxonomic characteristics of ZYC-3 T and BH-SS-21 T were defined using a polyphasic approach. The analysis of the full-length 16S rRNA gene sequence revealed that ZYC-3 T and BH-SS-21 T represented members of the genus Streptomyces . ZYC-3 T had less than 98.7% sequence similarities to all species of the genus Streptomyces , while BH-SS-21 T exhibited 99.97, 98.95, 98.83, 98.82, 98.75 and less than 98.7% sequence similarities to ' Streptomyces dioscori ' A217, Streptomyces ederensis JCM 4958 T , Streptomyces glomeroaurantiacus NBRC 15418 T , Streptomyces aurantiacus NBRC 13017 T , Streptomyces umbrinus JCM 4521 T and other species with validly published names in the genus Streptomyces . However, the digital DNA–DNA relatedness and average nucleotide identity values between ZYC-3 T , BH-SS-21 T , and their closely related strains were significantly lower than the recommended threshold values. Also, phenotypic, chemotaxonomic and genetic features distinguished ZYC-3 T and BH-SS-21 T from their reference strains. On the basis of their genotypic and phenotypic characteristics, strains ZYC-3 T and BH-SS-21 T were classified as representing novel species of the genus Streptomyces under the names Streptomyces liliifuscus sp. nov. ZYC-3 T (=CICC 25040 T =JCM 34560 T =MCCC 1K04978 T ) and Streptomyces liliiviolaceus sp. nov. BH-SS-21 T (=MCCC 1K06236 T =JCM 34767 T ), respectively.