Abstract 3540: Overexpression of incrna h19 enhances development and metastasis of gastric cancer

Abstract Background: Long non-coding RNAs (lncRNAs) have been shown to play key roles in the progression and metastasis in some carcinomas in recent studies. We found that the expression of long non-coding RNA H19 (H19) was much higher in gastric cancer tissues than that in paired noncanerous tissues. But its mechanism in gastric cancer remains unknown. The object of this study is to explore the role of H19 and it derived miR-675 in gastric cancer and to discover the downstream target genes. Methods: We detected the expression of H19 in 74 gastric tissues together with paired noncancerous tissues and followed-up median 27 months. Then, we investigated the role of H19 and miR-675 by knock-down their expression in MKN45 cell line and ectopic expression in SGC7901 cell line in vitro and in vivo. Finally, we built the co-expression network of H19 and demonstrate the target genes by RIP and DLR. Results: The overall survival (OS) in the patients with low H19 expression (ratio<6) is much longer than that with high H19 expression (ratio≥6). Overexpression of H19 can enhance the characteristics of gastric cancer cells such as proliferation, migration, invasion Tumor formation and metastasis. We built the co-expression network of H19 and demonstrated ISM1 was the binding protein of H19. The expression of ISM1 was positive correlated with that of H19. CALN1 was the target gene of miR-675. The expression of CALN1 was negative correlated with that of miR-675. Conclusion: Overexpression of H19 correlates with bad prognosis in gastric cancer. H19 acts as an oncogene in gastric cancer to enhance the proliferation, migration, invasion, and metastasis. MiR-675 also can enhance gastric cancer progression and metastasis. In additional, H19 RNA is actively bind to protein ISM1 however miR-675 targets the protein CALN1. These suggested that H19 effect on GC both through encoding miR-675 and up-regulation ISM1. Citation Format: Jiangfang Li, Hao Li, Liping su, Zheng-gang Zhu, Bingya Liu. Overexpression of incrna h19 enhances development and metastasis of gastric cancer. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3540. doi:10.1158/1538-7445.AM2014-3540