Triple-helix polysaccharides: Formation mechanisms and analytical methods

Triple helix conformation of polysaccharides is generally believed to be associated with their biological activities. However, the formation mechanisms and analytical methods for the triple helix polysaccharides, to our best knowledge, have not been systematically investigated. This article reviews specifically the literature on the formation and characterization of triple-helix polysaccharides. The formation mechanisms and related structural-conformation-bioactivity relationships are discussed; various analytical methods for characterizing triple-helix polysaccharides are summarized. This review devotes to a better understanding of the formation of polysaccharides based triple-helix structure and related analytical methods. These could provide some insights and inspirations for their applications in both food and pharmaceutical industries.