Determination of Three Flavonoids In Seeds of Astragali Complanati by HPLC

Objective:To estabish the HPLC method for determining the content of three flavonoids(calycosin,formononetin and rhamnocitrin)in the seeds of Astragali complanati.Method:An HPLC method has been developed to separate the three flavonoids on Kromasil C18(4.6 mm×250 mm,5 μm)column with methanol-1% acetic acid(56∶44)as mobile phase(1 mL·min-1),column temperature at 30 ℃ and UV detection wavelength at 266 nm.Result:The good linearity range of calycosin,formononetin and rhamnocitrin is 0.348-1.74(r=0.999 7),0.288-1.44(r=0.999 8),0.120-0.60(r=0.999 7).The average recovery of Calycosin,Formononetin and Rhamnocitrin are 100.3%(RSD 0.33%),99.8%(RSD 2.78%),98.6%(RSD 2.91%).Conclusion:The estabished method is simple,accurate and can be used for simultaneously determining the content of three flavonoids and for the the quality control of the seeds of A.complanati.