Sex hormone-binding globulin has no effect on salivary testosterone

We would like to follow up our 2014 publication in this journal (1) by submitting further validation data to support the measurement of salivary testosterone (T), highlighting its independence from variations in circulating sex hormone-binding globulin (SHBG), unlike serum total T which is positively correlated with SHBG.All analyses were performed as previously described (1).In addition, further samples were collected from, 48 males and 38 females, healthy volunteers (mean age 32 y, range 18-66).Ethical committee approval was granted from Ghent University Hospital.These samples were analysed for salivary T, total T and equilibrium dialysis using liquid chromatography-tandem mass spectrometry (2).