化学
铁蛋白
壳聚糖
美拉德反应
没食子酸
没食子酸表没食子酸酯
胰蛋白酶
药物输送
吸收(声学)
动态光散射
核化学
色谱法
生物化学
有机化学
纳米颗粒
多酚
抗氧化剂
纳米技术
酶
材料科学
复合材料
作者
Rui Yang,Yuqian Liu,Yunjing Gao,Yongjin Wang,Christopher L. Blanchard,Zhongkai Zhou
标识
DOI:10.1016/j.ijbiomac.2017.07.040
摘要
Ferritin is a shell-like carrier protein with an 8 nm diameter cavity which endows a natural space to encapsulate food and drug components. In this work, phytoferritin was unprecedentedly glycosylated by chitosan to fabricate ferritin-chitosan Maillard reaction products (FCMPs) (grafting degree of 26.17%, 24 h, 55 °C). Results indicated that the amide I and II bands of ferritin were altered due to the chitosan grafting, whereas the ferritin spherical structure were retained. Simulated digestion analysis showed that the FCMPs were more resistant to pepsin and trypsin digestion as compared with ferritin alone. Furthermore, FCMPs were employed as carrier to encapsulate epigallocatechin gallate (EGCG) molecules with an encapsulation ratio of 12.87% (w/w), and the resulting FCMPs-EGCG complexes showed a slow release of EGCG in simulated gastrointestinal tract. Interestingly, different types of food components displayed different effects in EGCG release behavior from the FCMPs, wherein proanthocyanidin, milk and soy protein inhibited the EGCG release. In addition, the absorption of EGCG encapsulated in FCMPs in Caco-2 monolayer model was significantly improved as compared with free EGCG. This work provides a novel nano-vehicle for fabricating core-shell systems in food and drug delivery domain.
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