A study on β-lactamase activity of biofilm Escherichia coli

Objective To investigate the mechanism of drug resistance of biofilm escherichia coli .Methods The model of escherichia coli biofilm was established with the flat board method And the biofilm was confirmed by scanning electron microscopy The β lactamase activities were quantitated in escherichia coli, biofilm escherichia coli , biofilm escherichia coli induced by impenem or cefoxitin. Results The β lactamase activity of biofilm escherichia coli was 2 16 times as much as that of escherichia coli planctonically, and the β lactamase activities of biofilm escherichia coli induceded by impenem or cefoxitin were 1 30 and 1 05 times as much as those of biofilm escherichia coli ,respectively.Conclusion The drug resistance to antibiotics of biofilm escherichia coli was related to the production of β lactamase.