再生(生物学)
心肌梗塞
心室
医学
梗塞
心室
生物
生物信息学
细胞生物学
病理
心脏病学
作者
Ahmed I. Mahmoud,Enzo R. Porrello,Wataru Kimura,Eric N. Olson,Hesham A. Sadek
出处
期刊:Nature Protocols
[Springer Nature]
日期:2014-01-16
卷期号:9 (2): 305-311
被引量:143
标识
DOI:10.1038/nprot.2014.021
摘要
Although amphibian and fish models of heart regeneration have existed for decades, a mammalian equivalent has long remained elusive. Our discovery of a brief postnatal window for heart regeneration in neonatal mice has led to the establishment of surgical models for cardiac regenerative studies in mammals for the first time. This protocol describes a 10-min surgical procedure to induce cardiac injury in 1-d-old neonatal mice. This allows for the analysis of cardiac regeneration after surgical amputation of the left ventricle (LV) (apical resection) and coronary artery occlusion (myocardial infarction (MI)). A comparative analysis of neonatal and adult responses to myocardial injury should enable identification of the key differences between regenerative and nonregenerative responses to cardiac injury. This protocol can also be adapted to the growing repertoire of genetic models available in the mouse, and it provides a valuable tool for unlocking the molecular mechanisms that guide mammalian heart regeneration during early postnatal life.
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