同源盒
生物
EMX2型
同源框蛋白Nkx-2.5
同源框A1
遗传学
表型
LIM域
基因
领域(数学分析)
C端
终端(电信)
转录因子
氨基酸
计算机科学
计算机网络
数学分析
数学
锌指
作者
Kyle Serikawa,Patricia Zambryski
出处
期刊:Plant Journal
[Wiley]
日期:1997-04-01
卷期号:11 (4): 863-869
被引量:23
标识
DOI:10.1046/j.1365-313x.1997.11040863.x
摘要
Domain exchange constructs that traded regions surrounding the homeodomain were constructed for two kn1-like genes, KNAT1 and KNAT3, and introduced into Arabidopsis thaliana under the control of the 35S CaMV promoter. The kn1-like homeodomain proteins all have the homeodomain located near the C-terminus of the protein, and also share a second conserved domain (the ELK domain) immediately N-terminal to the homeodomain. Progeny were scored for the appearance of the KNAT1 overexpression phenotype. A construct containing the KNAT3 N-terminus and the KNAT1 ELK- and homeodomain resulted in a KNAT1 overexpression phenotype, indicating that specificity mainly resides within the ELK- and homeodomain region. Further exchanges demonstrated that specificity probably does not arise from a single region within the ELK and/or homeodomain but rather requires sequences both N-terminal and C-terminal to residue 23 of the homeodomain. Further, in contrast to some animal homeodomains, KNAT1 does not utilize the residues of the N-terminal arm of the homeodomain for specificity.
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