生物制造
生物加工
喷嘴
制作
材料科学
微流控
生物医学工程
过程(计算)
纳米技术
微电子机械系统
组织工程
计算机科学
机械工程
工程类
医学
生物
病理
操作系统
遗传学
替代医学
作者
Robert C. Chang,Jae Do Nam,Wei Sun
出处
期刊:Tissue Engineering Part A
[Mary Ann Liebert]
日期:2008-01-01
卷期号:14 (1): 41-48
被引量:425
标识
DOI:10.1089/ten.a.2007.0004
摘要
Novel technologies are emerging that incorporate cells as part of the building blocks for various biomanufacturing processes, such as solid freeform fabricated tissue constructs for tissue regeneration, three-dimensional pharmacokinetic models, cell-based microelectromechanical systems, sensors, and microfluidic devices. However, the effects of these biomanufacturing processes on cells have not been fully studied. This paper examines the effect of solid freeform fabrication–based direct cell writing process, focusing on dispensing pressure and nozzle size, on the viability and functional behavior of HepG2 cells encapsulated within alginate. Our experimental results revealed a process-induced mechanical damage to cell membrane integrity, causing a quantifiable loss in cell viability due to incremental increases and decreases in the studied process parameters of dispensing pressure and nozzle size, respectively. The experimental results also suggested that cells may require a recovery period following direct cell writing biofabrication. The general finding of this study may be applicable to freeform fabrication of cell-based tissue constructs and three-dimensional biological models.
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