Fabrication and characterization of the nano-composite of whey protein hydrolysate chelated with calcium

The chelation of calcium ions to WPH caused molecular folding and aggregation leading to the formation of a WPH-calcium chelate of nanoparticle size, and the principal sites of calcium-binding corresponded to the carboxyl groups and carbonyl groups of WPH. The WPH-calcium chelate could significantly improve calcium absorption at the intestinal level.