Modification of rGO/ZIF-68 Based on Molecularly Imprinted Polymer Electrochemical Sensor for Detection of Acetamiprid

Molecular imprinting (MIP) is one of the most efficient strategies used to provide recognition materials with continuous good selectivity, commonly employed in electrochemical sensors as recognition elements or modifier agents[1]. In this study, a highly sensitive Molecularly Imprinted Polymer (MIP) sensor was developed and successfully utilized for acetamiprid recognition. The MIP was decorated onto the surface of a zeolitic imidazolate framework (ZIF-68) and reduced graphene oxide (rGO) composite modified with a glassy carbon electrode (GCE) to fabricate the GCE/rGO/ZIF-68/MIP electrode. Spectroscopic and microscopic analyses such as XRD, FT-IR, XPS, and SEM were employed to evaluate the composition and morphology of the surface of the GCE/rGO/ZIF-68/MIP electrode. The electrochemical characterization of the electrodes was performed using cyclic voltammetry (CV), Squarewave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Additionally, different parameters influencing the sensitivity of GCE/rGO/ZIF-68/MIP, such as the percentage of rGO, template:monomer ratio, number of electropolymerization cycles, pH, extraction and incubation time were optimized. Under optimal conditions, the MIP sensor exhibited a wide linear range, low detection limit, as well as good reproducibility, stability, and selectivity, and it was successfully used for the determination of pesticide in real solutions. The sensor was applied to real samples for the acetamiprid pesticide, and successful recovery values were obtained. Keywords: Electrochemical sensor; Molecularly imprinted polymer (MIP); Acetamiprid.