试剂
荧光
选择性
化学
联氨(抗抑郁剂)
亲核细胞
组合化学
发色团
体内
光化学
色谱法
有机化学
物理
催化作用
生物技术
量子力学
生物
作者
Yan Shi,Fangjun Huo,Caixia Yin
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:148 (15): 3491-3497
被引量:2
摘要
Hydrazine (N2H4) is an important reagent in the field of fine chemical engineering. However, its accumulation in the environment and food chain could pose a great threat to food safety and human health. Therefore, designing a fluorescent probe with good cell penetration and high selectivity and sensitivity to detect N2H4 in actual samples and in vivo is a meaningful project. Herein, due to the nucleophilicity of hydrazine, we utilized naphthalimide as the fluorescence chromophore and pyrone as the recognition site to achieve the ratiometric detection of hydrazine by ring opening. In addition, we introduced the ester to improve the lipid solubility of the probe, which allowed the probe to better penetrate the cell membrane to realize the fluorescent imaging of probes in cells. Meanwhile, to our delight, the probe showed high selectivity and sensitivity to N2H4 in the test system, so we further applied the probe in water samples and food, in vitro and in vivo.
科研通智能强力驱动
Strongly Powered by AbleSci AI