已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Dengue virus infection induces selective expansion of Vγ4 and Vγ6TCR γδ T cells in the small intestine and a cytokine storm driving vascular leakage in mice

登革热病毒 登革热 细胞激素风暴 封锁 免疫学 细胞因子 生物 促炎细胞因子 肿瘤坏死因子α 干扰素 炎症 受体 医学 内科学 生物化学 疾病 2019年冠状病毒病(COVID-19) 传染病(医学专业)
作者
Takeshi Kurosu,Daisuke Okuzaki,Yusuke Sakai,Mohamad Al Kadi,Supranee Phanthanawiboon,Yasusi Ami,Masayuki Shimojima,Tomoki Yoshikawa,Shuetsu Fukushi,Noriyo Nagata,Tadaki Suzuki,Daisuke Kamimura,Masaaki Murakami,Hideki Ebihara,Masayuki Saijo
出处
期刊:PLOS Neglected Tropical Diseases 卷期号:17 (11): e0011743-e0011743
标识
DOI:10.1371/journal.pntd.0011743
摘要

Dengue is a major health problem in tropical and subtropical regions. Some patients develop a severe form of dengue, called dengue hemorrhagic fever, which can be fatal. Severe dengue is associated with a transient increase in vascular permeability. A cytokine storm is thought to be the cause of the vascular leakage. Although there are various research reports on the pathogenic mechanism, the complete pathological process remains poorly understood. We previously reported that dengue virus (DENV) type 3 P12/08 strain caused a lethal systemic infection and severe vascular leakage in interferon (IFN)-α/β and γ receptor knockout mice (IFN-α/β/γRKO mice), and that blockade of TNF-α signaling protected mice. Here, we performed transcriptome analysis of liver and small intestine samples collected chronologically from P12/08-infected IFN-α/β/γRKO mice in the presence/absence of blockade of TNF-α signaling and evaluated the cytokine and effector-level events. Blockade of TNF-α signaling mainly protected the small intestine but not the liver. Infection induced the selective expansion of IL-17A-producing Vγ4 and Vγ6 T cell receptor (TCR) γδ T cells in the small intestine, and IL-17A, together with TNF-α, played a critical role in the transition to severe disease via the induction of inflammatory cytokines such as TNF-α, IL-1β, and particularly the excess production of IL-6. Infection also induced the infiltration of neutrophils, as well as neutrophil collagenase/matrix metalloprotease 8 production. Blockade of IL-17A signaling reduced mortality and suppressed the expression of most of these cytokines, including TNF-α, indicating that IL-17A and TNF-α synergistically enhance cytokine expression. Blockade of IL-17A prevented nuclear translocation of NF-κB p65 in stroma-like cells and epithelial cells in the small intestine but only partially prevented recruitment of immune cells to the small intestine. This study provides an overall picture of the pathogenesis of infection in individual mice at the cytokine and effector levels.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
luochen完成签到,获得积分10
9秒前
14秒前
zhangweiqiao完成签到,获得积分10
14秒前
鱼儿游完成签到 ,获得积分10
14秒前
河鲸完成签到 ,获得积分10
16秒前
Amancio118完成签到 ,获得积分10
17秒前
猫的淡淡完成签到,获得积分10
17秒前
蜗牛星星完成签到,获得积分10
19秒前
辛夷完成签到,获得积分10
21秒前
affff完成签到 ,获得积分10
23秒前
谨慎长颈鹿完成签到,获得积分10
25秒前
执念完成签到 ,获得积分10
25秒前
岸在海的深处完成签到 ,获得积分10
27秒前
王奥飞完成签到 ,获得积分10
28秒前
小宋完成签到,获得积分10
28秒前
舒心的寻琴完成签到,获得积分10
29秒前
hello2001完成签到 ,获得积分10
29秒前
30秒前
司空致远完成签到,获得积分10
34秒前
37秒前
毛肚吃不腻完成签到 ,获得积分10
37秒前
cctv18应助zhangweiqiao采纳,获得10
38秒前
司空致远发布了新的文献求助10
39秒前
布可完成签到,获得积分10
39秒前
CipherSage应助科研通管家采纳,获得10
39秒前
科研通AI2S应助科研通管家采纳,获得10
39秒前
田様应助科研通管家采纳,获得10
39秒前
NexusExplorer应助科研通管家采纳,获得10
39秒前
赘婿应助科研通管家采纳,获得10
39秒前
SOLOMON应助科研通管家采纳,获得10
39秒前
CharlotteBlue应助科研通管家采纳,获得30
40秒前
小二郎应助科研通管家采纳,获得30
40秒前
宇文非笑完成签到 ,获得积分10
41秒前
chengguan发布了新的文献求助10
42秒前
然后关注了科研通微信公众号
44秒前
deswin完成签到 ,获得积分10
47秒前
49秒前
51秒前
自信放光芒~完成签到 ,获得积分10
52秒前
lin完成签到 ,获得积分10
53秒前
高分求助中
Formgebungs- und Stabilisierungsparameter für das Konstruktionsverfahren der FiDU-Freien Innendruckumformung von Blech 1000
The Illustrated History of Gymnastics 800
The Bourse of Babylon : market quotations in the astronomical diaries of Babylonia 680
Division and square root. Digit-recurrence algorithms and implementations 500
Elgar Encyclopedia of Consumer Behavior 300
機能營養學前瞻(3 Ed.) 300
Improving the ductility and toughness of Fe-Cr-B cast irons 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2509254
求助须知:如何正确求助?哪些是违规求助? 2159675
关于积分的说明 5529452
捐赠科研通 1879981
什么是DOI,文献DOI怎么找? 935492
版权声明 564161
科研通“疑难数据库(出版商)”最低求助积分说明 499472