光合作用
生物
生物量(生态学)
产量(工程)
糖
叶绿素
谷氨酰胺合成酶
氮气
鲁比斯科
芸苔属
类胡萝卜素
过氧化氢
铵
淀粉
磷酸烯醇式丙酮酸羧化酶
植物
碳纤维
光合效率
光系统
农学
光系统II
食品科学
硝酸还原酶
园艺
光合能力
作物
脯氨酸
氮气循环
APX公司
新陈代谢
作者
Mamta,Shiv Shanker Pandey,Anish Kaachra,Sachin Vashisath,Dinesh Kumar,Sanjay Kumar
摘要
Coexpression of genes involved in carbon (C) and nitrogen (N) metabolism offers a promising avenue for improving crop yield. This study investigated the impact of coexpressing phosphoenolpyruvate carboxylase [ZmPEPC (P)], aspartate aminotransferase [GmAspAT (A)] and glutamine synthetase [NtGS (G)] in Brassica juncea to enhance plant yield through improved C and N utilization. Plants coexpressing three genes (BjPAG) showed a significant increase in plant growth, biomass, chlorophyll and carotenoid content, while exhibiting decreased anthocyanin and hydrogen peroxide production. These improvements were observed under both ambient (~400 ppm; ACO2) and low (~200 ppm; LCO2) atmospheric CO2 conditions. Chlorophyll fluorescence study revealed that BjPAG plants had higher photosynthetic efficiency, particularly increased maximum quantum yield of PSII and performance index. This facilitated increased CO2 assimilation, total soluble sugar and starch accumulation, along with altered stomatal traits under both ACO2 and LCO2 conditions. Additionally, BjPAG plants accumulated less ammonium than control plants under both CO2 levels, indicating effective functioning of the overexpressed genes. Improved performance (enhanced photosynthetic rates, biomass and seed yield) of BjPAG plants under various N environments (6, 3 and 0.3 mm N) also supported improved N utilization. NMR analysis suggested that PAG-coexpression resulted in a higher flux of assimilated CO2 and NH3 towards sugars, amino acids, organic acids, polyphenols and glucosinolates. In conclusion, this study highlights the effectiveness of coexpressing ZmPEPC, GmAspAT and NtGS in B. juncea, leading to efficient C and N utilization, ultimately resulting in enhanced photosynthesis, plant biomass and seed yields, and as a promising strategy for increasing the yield of food crops.
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