拟南芥
生物
小RNA
细胞生物学
晶片切割
突变体
生物化学
化学
基因
有机化学
图层(电子)
作者
Gaozhan Zhao,Jinrong Niu,Zhuo-Yan Hai,Tengfei Li,Dixiang Xie,Yan Li,Yijun Qi
标识
DOI:10.1073/pnas.2305244120
摘要
MicroRNAs (miRNAs) play an important role in gene regulation. In Arabidopsis , mature miRNAs are processed from primary miRNA transcripts by the Dicing complex that contains Dicer-like 1 (DCL1), SERRATE (SE), and Hyponastic Leaves 1 (HYL1). The Dicing complex can form nuclear dicing bodies (D-bodies) through SE phase separation. Here, we report that Cyclophilin71 (CYP71), a peptidyl-prolyl isomerase (PPIase), positively regulates miRNA processing. We show that CYP71 directly interacts with SE and enhances its phase separation, thereby promoting the formation of D-body and increasing the activity of the Dicing complex. We further show that the PPIase activity is important for the function of CYP71 in miRNA production. Our findings reveal orchestration of miRNA processing by a cyclophilin protein and suggest the involvement of peptidyl-prolyl cis–trans isomerization, a structural mechanism, in SE phase separation and miRNA processing.
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