电化学发光
发光体
化学
公制(单位)
计算机科学
纳米技术
材料科学
发光
光电子学
电极
运营管理
物理化学
经济
作者
Julie Descamps,Camille Colin,Gilles Tessier,Stéphane Arbault,Nešo Šojić
标识
DOI:10.1002/anie.202218574
摘要
Here we report on a label-free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations of the [Ru(bpy)3 ]2+ luminophore. This work addresses the central point of the minimal concentration of the ECL luminophore required to image single entities. We demonstrate the possibility to record ECL images of cells and mitochondria at concentrations down to nM and pM. This is 7 orders of magnitude lower than classically-used concentrations and corresponds to a few hundreds of luminophores diffusing around the biological entities. Yet, it produces remarkably sharp negative optical contrast ECL images, as demonstrated by structural similarity index metric analyses and supported by predictions of the ECL image covering time. Finally, we show that the reported approach is a simple, fast, and highly sensitive method, which opens new avenues for ultrasensitive ECL imaging and ECL reactivity at the single molecule level.
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