O-Glycosylated RNA Identification and Prediction by Solid-Phase Chemoenzymatic TnORNA Method and PONglyRNA Tool

-glycosylation were identified, indicating the potential co-occurrence of N-linked and O-linked glycosylation on small RNAs. We have also developed PONglyRNA, an online bioinformatic tool for the prediction of glycosylated RNAs, which showed competitive performance in validation tests and potentials in improving glycosylation site prediction (http://ponglyrna.797000.xyz:8880/). In conclusion, this study establishes robust experimental and computational tools for identifying O-linked glycoRNAs. Additionally, it uncovers the novel role of glycosylation in PDAC development and progression through altered glycosylation of oncogenic miRNAs.