Tn3转座子
生物
整合酶
位点特异性重组
重组酶
整合酶
遗传学
突触
转座因子
生物化学
DNA
染色体
重组
基因组
基因
作者
Phalguni Ghosh,Amy I. Kim,Graham F. Hatfull
出处
期刊:Molecular Cell
[Elsevier]
日期:2003-11-01
卷期号:12 (5): 1101-1111
被引量:124
标识
DOI:10.1016/s1097-2765(03)00444-1
摘要
Integration of the mycobacteriophage Bxb1 genome into its host chromosome is catalyzed by a serine-integrase, a member of the transposon-resolvase family of site-specific recombinases. These enzymes use a concerted mechanism of strand exchange involving double-stranded cleavages with two-base extensions, and covalent protein-DNA linkages via phosphoserine bonds. In contrast to the resolvase/invertase recombination systems--where there are strict requirements for a specific synaptic complex within which the catalytic potential of the enzyme is activated--synapsis of attP and attB by Bxb1 integrase is completely promiscuous, aligning the sites with equal proclivity in parallel and antiparallel alignments. Moreover, the catalytic potential of Bxb1 integrase is fully active in either alignment. As a consequence, the nonpalindromic central dinucleotide (5'-GT) at the center of attP and attB is the sole determinant of Bxb1 prophage orientation, and a single base pair substitution in the two sites is sufficient to eliminate orientation control.
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