纳米笼
连接器
药物输送
体内
生物发光成像
癌细胞
纳米颗粒
铁蛋白
化学
纳米技术
材料科学
生物物理学
癌症
生物化学
生物
医学
荧光素酶
计算机科学
基因
生物技术
催化作用
内科学
操作系统
转染
作者
Melissa Bellini,Beatrice Riva,Veronica Tinelli,Maria Antonietta Rizzuto,Lucia Salvioni,Miriam Colombo,Francesca Mingozzi,Alberto Visioli,Laura Marongiu,Gianni Frascotti,Michael S. Christodoulou,Daniele Passarella,Davide Prosperi,Luisa Fiandra
出处
期刊:Small
[Wiley]
日期:2020-08-28
卷期号:16 (39)
被引量:30
标识
DOI:10.1002/smll.202001450
摘要
Abstract The identification of a highly sensitive method to check the delivery of administered nanodrugs into the tumor cells is a crucial step of preclinical studies aimed to develop new nanoformulated cures, since it allows the real therapeutic potential of these devices to be forecast. In the present work, the ability of an H‐ferritin (HFn) nanocage, already investigated as a powerful tool for cancer therapy thanks to its ability to actively interact with the transferrin receptor 1, to act as an efficient probe for the monitoring of nanodrug delivery to tumors is demonstrated. The final formulation is a bioluminescent nanoparticle, where the luciferin probe is conjugated on nanoparticle surface by means of a disulfide containing linker (Luc‐linker@HFn) which is subjected to glutathione‐induced cyclization in tumor cell cytoplasm. The prolonged imaging of luciferase+ tumor models, demonstrated by an in vitro and an in vivo approach, associated with the prolonged release of luciferin into cancer cells by disulfide bridge reduction, clearly indicates the high efficiency of Luc‐linker@HFn for drug delivery to the tumor tissues.
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