Analysis of different Jobstears Coix species with genomic in situ hybridization

Genomic in situ hybridization(GISH)method was used to study the relationship of different Jobstears Coix species,and the cell metaphase chromosomes of Coix Lacryma-jobi L,Coix Agrestis Lour and Coix Aquatica Roxb were hybridized with labelled probes genome from Coix Lacryma-jobi L.(AABB genomes).The GISH results indicated that the chromosomes of Coix Lacryma-jobi L and Coix Agrestis Lour was labelled with strong and intensive hybridization signals,which showed that the homology between Coix Lacryma-jobi L and Coix Agrestis Lour genomes was very close at genomic chromosomes level,and the conservative repeated sequences accounted for a large proportion.Moreover,the DNA components of 20 chromosomes from Coix Aquatica Roxb genome was highly homologic with the genome DNA of Coix Lacryma-jobi L.It is concluded that testing Coix aquatica Roxb belongs to hexaploid Coix aquatica Roxb population in Guangxi.