In cultivation of Agerocybe Dian A3×s,the Eupatorium adenophorum were used as material,at the same time,the straw and cotton seed hull were used as CK.The results showed that the formula which was mainly composed with Eupatorium adenophorum was the best,and the biological efficency could improve above 80%.The different effect between fresh and dry Eupatorium adenophorum on yield was not obvious.This opens a new way for control,extermination and utilization of Eupatorium adenophorum,and a new formula for cultivation of Agerocybe was offered.