黄芪
赭曲霉毒素A
流量(数学)
纳米颗粒
化学
材料科学
纳米技术
生物
植物
物理
真菌毒素
医学
机械
替代医学
病理
中医药
作者
Yangjiayi Xiang,Gou Wu,Cong Hu,Hongmei Li,Yan Li
标识
DOI:10.1016/j.snb.2024.135545
摘要
The prevalence and significant health risks associated with mycotoxin contamination in traditional Chinese medicines underscore the need for on-site detection methods. The complexity of matrices necessitates sample pretreatment before analysis, leading to prolonged analysis times and intricate procedures. In this study, we employed aptamer-modified magnetic nanoparticles (aptamer-MNPs) as labels and devised a three-in-one lateral flow aptasensor for on-site detection of ochratoxin A (OTA) in Astragalus membranaceus. By integrating aptamer-MNPs that serve in recognition, separation, and signal amplification roles, we streamlined purification, enrichment, and detection within a single aptasensor in 30 minutes. The developed aptasensor demonstrated high sensitivity (0.053 ng/mL), excellent specificity, and satisfactory accuracy. Analyzing twelve actual samples revealed three as OTA-positive, aligning well with HPLC analysis results. These results showcase the potential of the lateral flow aptasensor as a cost-effective, reliable, and robust on-site screening technique for trace-level mycotoxins in complex matrices.
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