平原的
RNA干扰
基因敲除
细胞生物学
表型
生物
乙醇
再生(生物学)
基因
模式生物
核糖核酸
遗传学
生物化学
作者
Olivier Cochet‐Escartin,Jason A. Carter,Milena Chakraverti-Wuerthwein,Joydeb Sinha,Eva‐Maria S. Collins
出处
期刊:Physical Biology
[IOP Publishing]
日期:2016-09-09
卷期号:13 (5): 055001-055001
被引量:12
标识
DOI:10.1088/1478-3975/13/5/055001
摘要
When freshwater planarians are exposed to a low-percentage (0.5%-1%) alcohol solution, they display a characteristic 'drunken' phenotype. Here we show that this drunken phenotype is a mixture of cilia-mediated gliding and scrunching, a muscular-based planarian gait which we recently demonstrated to be triggered by adverse environmental stimuli. At exogenous ethanol concentrations ≥2% (v/v), planarians become gradually immobilized and ultimately die. Using RNA interference (RNAi) for targeted gene knockdown, we elucidate the molecular basis for ethanol sensing and show that the big potassium ion channel SLO1 is necessary for ethanol sensitivity in planarians. Because slo1(RNAi) animals maintain their ability to scrunch in response to other adverse triggers, these results suggest that slo1 specifically regulates ethanol sensitivity and not the scrunching gait per se. Furthermore, this study demonstrates the ease of performing pharmacological studies in planarians. Combined with the worms' amenability to quantitative behavioral assays and targeted gene knockdown, planarians are a valuable model organism for studying the effect of neuroactive compounds on brain function and behavior.
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