Alpha Amylase Production by Aspergillus Oryzae Using Solid State Fermentation

A fungal strain of Aspergillus oryzae was used for the production of alpha amylase by solid state fermentation from agro-industrial waste. Enzyme production was growth associated and maximum activity (8.23 U/ml) were obtained after 120h when incubated at 30°C on wheat bran with initial moisture content 60%; initial medium pH = 5 . Enzyme activity increased when the solid medium was supplemented with additional nitrogen source (sodium Nitrate).