细胞凋亡
癌症研究
免疫印迹
细胞生物学
足细胞
流式细胞术
阿布勒
酪氨酸激酶
医学
内科学
生物
信号转导
分子生物学
生物化学
肾
蛋白尿
基因
作者
Yiqiong Ma,Qian Yang,Xinghua Chen,Wei Liang,Zhigang Ren,Guohua Ding
标识
DOI:10.1016/j.diabres.2015.12.013
摘要
To investigate the role of the non-receptor tyrosine kinase c-Abl in high glucose-induced podocyte injury and its possible signal transduction pathway.Sixteen C57BL/6 mice were randomly assigned to a group with diabetes and a normal control group. Subsequently, differentiated mouse podocytes were exposed to high-glucose conditions, and podocyte apoptosis was then assessed by flow cytometry and Hoechst 33258 staining. Western blot and immunofluorescence assay were used to measure c-Abl expression. Co-immunoprecipitation assay was used and c-Abl siRNA was applied to evaluate the interaction between c-Abl and p53.High glucose promotes podocyte apoptosis. The c-Abl expression in podocytes was increased after exposure to high glucose, stimulating the p53 signaling pathway. Conversely, treatment with c-Abl siRNA restored high glucose-promoted podocyte apoptosis and resulted in the reduction of p53 expression.c-Abl contributes to high glucose-induced podocyte apoptosis via p53 signaling pathway.
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