机械转化
材料科学
基质(水族馆)
生物物理学
组织工程
再生医学
干细胞
粘附
纳米技术
细胞粘附
机械生物学
细胞生物学
生物
生态学
遗传学
复合材料
作者
Jun‐Sheng Zhang,Siu Hong Dexter Wong,Xin Wu,Hong Lei,Meng Qin,Peng Shi,Wei Wang,Liming Bian,Yi Cao
标识
DOI:10.1002/adma.202105765
摘要
Regulating stem cell functions by precisely controlling the nanoscale presentation of bioactive ligands has a substantial impact on tissue engineering and regenerative medicine but remains a major challenge. Here it is shown that bioactive ligands can become mechanically "invisible" by increasing their tether lengths to the substrate beyond a critical length, providing a way to regulate mechanotransduction without changing the biochemical conditions. Building on this finding, light switchable tethers are rationally designed, whose lengths can be modulated reversibly by switching a light-responsive protein, pdDronpa, in between monomer and dimer states. This allows the regulation of the adhesion, spreading, and differentiation of stem cells by light on substrates of well-defined biochemical and physical properties. Spatiotemporal regulation of differential cell fates on the same substrate is further demonstrated, which may represent an important step toward constructing complex organoids or mini tissues by spatially defining the mechanical cues of the cellular microenvironment with light.
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