Enrichment of Paeoniflorin in Common in Paeonia lactiflora and Paeonia suffruticosa by Macroporous Adsorption Resins

Objective:To search for the method used in enriching paeoniflorin in common in Paeonia lactiflora and Paeonia suffruticosa by macroporous absorption resin.Methods:HPLC method was used to determine the concentrations of Paeoniflorin.3 types of macroporous absorption resin were optimized,taking the absorption capacity and elution ratios as the criteria.The eluting conditions were optimized by using the orthogonal test,taking the elution ratios and product purity as the criteria.Results:The elution ratios and purity of Paeoniflorin were more than 90% and 35% respectively,following the optimized eluting conditions by the D-301 type macroporous absorption resin.Conclusion:The D-301 type macroporous absorption resin shows better comprehensive adsorption property. It is available for the enrichment of paeoniflorin in Paeonia lactiflora and Paeonia suffruticosa.