Study on the lanostane triterpenoids fromInonotus obliquus

Objective To study the lanostane tetracyclic triterpenoids in Inonotus obliquus.Methods The powered materials was extracted with 95% EtOH and the extracts was separated by petroleum ether,ethyl acetate and nbutanol successively to 4parts.The ethyl acetate extracts was futher isolated by silica gel,ODS,sephadex LH-20 and HPLC chromatograph to obtain the compounds.Their structures were identified by physicochemical properties and spectroscopy(1H-NMR,13C-NMR,DEPT,HSQC,HMBC,HHCOSY and NOESY).Results 6compounds were isolated from the ethyl acetate extracts of I.obliquus.They were identified as lanosterol(1),inotodiol(2),3βhydroxy-lanosta-8,24-diene-21-al(3),inonotsuoxide A(4),inonotsuoxide B(5)and trametenolic acid(6) respectively.Conclusion The chromatographical trait,physicochemical properties and spectroscopical pattern of the the compounds were analysised.DEPT and HSQC of inonotsuoxide A(4)and inonotsuoxide B(5)were reported for the first time.