SDS-PAGE Analysis of the Effectiveness on Serum Proteomics of Mouse Hepatoma Cell Line H22 by GASPGF

Objective:The technique and strategy of serum proteomics were used to differentiate and identify proteins secreted by mouse hepatoma cell line H22 and normal mouse.Through the variation in serum protein expression map,we can observe the effectiveness of GASPGF.Construct technique platform on proteomics for researching the traditional Chinese medicine to inhibit tumor.Methods:Supported by electrophoretic technique.Set up the serum protein expression map of all groups that included normal group,tumor group and GASPGF group.One dimensional SDS-PAGE electrophorsis was performed to separate the proteins of every group,searched differentially expressed protein spots or straps.Results:Normal group has 20 straps;tumor group has 24 straps;GASPGF group has 22 straps.Conclusion: GASPGF can affect the serum proteomics of mouse.